Running the gel:

1. Place 250 mL beaker on scale and add 1.25 g agarose. Then fill to 62.5g with .5x TBE buffer and heat in microwave for 1.5 min. Refill to 62.5 g with ddH2O and 0.5mL 1.375M MgCl2.

2. Carefully add 2 μL of ethidium bromide (EtBr) and mix the contents of the beaker thoroughly before pouring out into a gel rig. Place comb into gel rig and allow mixture to cool 15-20 mins before removing comb.

3. Fill the gel rig with 0.5 x TBE with 11mMmg buffer.

4. Load 17uL of samples into the wells.

5. Load 6uL of 100kbps ladder into the gel.

6. Secure the cathode and anode to the rig and power source, and run for 2 hours in 80 volts. Place ice around rig so that overheating does not occur.

7. Remove gel from rig and place it on the UV table.

8. Place the camera over the gel and turn the camera on.

9. Open the imaging software on the computer. Center camera over the gel and take the image.

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Preparing the samples:

1. Add 15uL of sample to 3uL of loading dye.

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References

Carlos E. Castro et al., A Primer to Scaffolded DNA Origami. Nature Methods 2011.