Deoxyribonucleic acid (DNA) nanotechnology has been developing rapidly in recent years through the introduction of various structural designs of DNA origami/tile assemblies. However, studies of DNA origami cell interactions are limited due to the complexity of cell structures. Synthetic cells can be used for studies of individual cell functions with external control mechanisms. This project aims to design a reconfigurable DNA origami 3-arm nanostructure as the basic building block of a hierarchical nanomachine assembly. The team has designed a 3-arm structure with a uniform length of 50 nm for each arm. The structure is reconfigurable by switching the connection profile between the three arms to a single-stranded, double-stranded, or cross-over connection. The reconfigurability of the 3-arm structure allows it to change the angle between arms, allowing the higher-order assembly to generate motion or ultimately change the structure's overall configuration. Future work for this project includes polymerization of the 3-arm structure and control of the system through reconfiguration. Potential applications of this nano-system include synthetic cell studies such as forming a contractile ring to assist in cell mitosis and membrane deformation in endo-/exocytosis. This structure can also be used for future research in non-cellular systems to create adaptive membranes for ionic channel.